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KMID : 0355620140400040173
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Journal of Korean Association of Oral and Maxillofacial Surgeons
2014 Volume.40 No. 4 p.173 ~ p.180
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Neurogenic differentiation of human dental stem cells in vitro
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Lee Joo-Hee
Um So-Youn
Song In-Seok
Kim Hui-Young
Seo Byoung-Moo
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Abstract
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Objectives: The purpose of this study was to investigate the neurogenic differentiation of human dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), and stem cells from apical papilla (SCAP).
Materials and Methods: After induction of neurogenic differentiation using commercial differentiation medium, expression levels of neural markers, microtubule-associated protein 2 (MAP2), class III β-tubulin, and glial fibrillary acidic protein (GFAP) were identified using reverse transcriptase polymerase chain reaction (PCR), real-time PCR, and immunocytochemistry.
Results: The induced cells showed neuron-like morphologies, similar to axons, dendrites, and perikaryons, which are composed of neurons in DPSCs, PDLSCs, and SCAP. The mRNA levels of neuronal markers tended to increase in differentiated cells. The expression of MAP2 and β-tubulin III also increased at the protein level in differentiation groups, even though GFAP was not detected via immunocytochemistry.
Conclusion: Human dental stem cells including DPSCs, PDLSCs, and SCAP may have neurogenic differentiation capability in vitro. The presented data support the use of human dental stem cells as a possible alternative source of stem cells for therapeutic utility in the treatment of neurological diseases.
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KEYWORD
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Dental pulp, Periodontal ligament, Dental papilla, Stem cells, Neurogenic differentiation
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